9.12–9.14How materials are cycled
Many different materials cycle through the abiotic and biotic components of an ecosystem. All materials in the living world are recycled to provide the building blocks for future organisms — the carbon atoms in you were in something else before, and will be again.
The carbon cycle returns carbon from organisms to the atmosphere as carbon dioxide, to be used by plants in photosynthesis.
The water cycle provides fresh water for plants and animals on land before draining into the seas. Water is continuously evaporated and precipitated.
You are not expected to study the nitrogen cycle.
The water cycle has four processes to name, and a question showing you a diagram will ask you to explain them rather than list them.
• Evaporation — energy from the Sun turns liquid water at the surface of the seas into water vapour. Plants add to this by transpiration, losing water vapour through their stomata. • Condensation — the vapour rises, cools, and condenses back into tiny droplets, forming clouds. • Precipitation — the droplets join until they are heavy enough to fall as rain, snow or hail, much of it onto land. • Runoff and collection — the water drains through the soil and along rivers, back into the seas, and the cycle repeats.
Why it matters to living organisms is the part most often left out: the cycle provides fresh water for plants and animals on land. Sea water is salty and unusable for most organisms, and evaporation leaves the salt behind — so the cycle is what turns an ocean into drinkable water, continuously, and delivers it inland.
The role of microorganisms is the part most often left out of an answer. Decomposers break down dead plants and animals and waste, returning carbon to the atmosphere as carbon dioxide through their own respiration, and returning mineral ions to the soil where plants can absorb them again. Without them the nutrients would stay locked in dead material and the cycle would stop.
9.15The nitrogen cycle
Plants need nitrogen to make amino acids and therefore proteins. The air is almost four-fifths nitrogen gas, and a plant can use none of it: the molecule is too unreactive. What a plant can absorb is nitrates, dissolved in water, taken up through the roots. Getting nitrogen out of the air and into a nitrate is the work of bacteria, and four groups do it — knowing which does what is most of this topic.
Lightning also fixes a small amount of nitrogen directly, and the whole cycle closes when animals eat plants, take in the plant protein, and return nitrogen to the soil in their waste and when they die.
Farmers work with the cycle in three ways. Fertilisers add nitrates directly, which is quick but expensive and washes into waterways. Crop rotation grows a legume such as clover or peas every few years, so the nitrogen-fixing bacteria in its root nodules restore the soil for free — and ploughing the crop back in adds the nitrogen it holds. Draining and ploughing land keeps the soil aerated, which favours the nitrifying bacteria that make nitrates and works against the denitrifying bacteria that destroy them.
Common mistake
Nitrogen-fixing and nitrifying bacteria are not the same thing, and swapping them is the most common error in this topic. Fixing takes nitrogen out of the air; nitrifying works on ammonia that is already in the soil.
9.17B–9.19BDecomposition
Biology only — not on the Combined Science specification
Three factors affect the rate of decay of biological material, and each acts on the microorganisms doing the decaying:
• Temperature — warmth speeds up the enzymes in the decomposers, up to an optimum; too hot and those enzymes denature, so decay slows again. • Water — decomposers need moisture to survive and to secrete their enzymes; dry material barely decays, which is why food is preserved by drying. • Availability of oxygen — most decomposers respire aerobically, so more oxygen means faster decay.
Gardeners and farmers try to provide optimum conditions for rapid decay of waste biological material. The compost produced is used as a natural fertiliser for growing garden plants or crops — which is why a compost heap is kept moist, turned to let air in, and works faster in summer.
Anaerobic decay produces methane gas. Biogas generators can be used to produce methane as a fuel — decay without oxygen, deliberately, so the gas can be collected and burned.
Required practical 10
Investigate the effect of temperature on the rate of decay of fresh milk by measuring pH change
- Add a fixed volume of fresh milk and a fixed volume of lipase to a test tube, with a few drops of cresol red indicator.
- Place the tube in a water bath at a set temperature and record the starting pH.
- Lipase breaks down the fats in milk into fatty acids, which lowers the pH — time how long it takes for the indicator to change colour.
- Repeat at a range of temperatures, keeping the volumes and concentrations the same.
- Calculate the rate as 1 ÷ time, and plot rate against temperature.
Watch out: Be clear about what the pH is telling you. Decay means large molecules being broken down by enzymes, and the products of breaking down milk are acidic — so a falling pH is a measure of how fast that breakdown is going. The faster the pH falls, the faster the decay. In the version using lipase, the enzyme is added directly and digests the fats into fatty acids. In real decay the same job is done by enzymes that microorganisms secrete into their surroundings, which is why the practical models decomposition rather than being it. Either way the temperature effect is the same and for the same reason: enzymes work faster as it warms, up to an optimum, and denature beyond it. Time and rate are inverses, so the shortest time is the fastest rate. Calculate rate changes as 1 ÷ time and the graph takes the familiar enzyme shape.
Handling decay data is examined alongside the biology. Calculate rate changes as 1 ÷ time, then translate the information between numerical and graphical form: temperature on the x-axis, rate on the y-axis, a scale for the axes that fills at least half the grid, and a smooth curve rather than joined dots. Reading back the other way, quote the rate at a stated temperature with its units, and describe the curve as rising to an optimum and then falling — the same enzyme shape as every other rate graph in the course.
Worked example
Milk takes 200 seconds to decay at 20 °C and 50 seconds at 40 °C. Calculate the rate at each temperature and compare them.
- Rate = 1 ÷ time.
- At 20 °C: 1 ÷ 200 = 0.005 s⁻¹.
- At 40 °C: 1 ÷ 50 = 0.02 s⁻¹.
- Compare: 0.02 ÷ 0.005 = 4.
Answer: 0.005 s⁻¹ and 0.02 s⁻¹ — the rate is four times faster at 40 °C, because the enzymes of the decomposers work faster at the higher temperature.
- Decomposition
- the breakdown of biological material by microorganisms, whose rate depends on temperature, water and the availability of oxygen
- Anaerobic decay
- decay without oxygen, which produces methane gas; biogas generators use it deliberately so the methane can be collected and burned as a fuel
9.16BIndicator speciesHigher tier
Biology only, and Higher tier only
Some species survive only within a narrow range of conditions. Because of that, finding them — or failing to find them — measures the conditions without any equipment at all. A species used this way is called an indicator species.
In water, the measure is usually dissolved oxygen, which falls when organic pollution such as sewage or fertiliser runoff feeds a bloom of decomposers. Bloodworms and sludgeworms tolerate very low oxygen levels, so finding them in numbers indicates polluted water. Stonefly nymphs and freshwater shrimp need high oxygen levels, so finding them indicates clean water.
In air, the measure is usually sulfur dioxide. Lichens are sensitive to it, so a wide variety of lichens on trees and walls indicates clean air, and bare bark indicates polluted air. Blackspot fungus on rose leaves behaves the same way — it disappears where the air is polluted.
Evaluating the method is what the higher-tier marks are usually for, so hold both sides ready. It is cheap, needs no equipment and no expertise beyond identification, and because the organisms have lived there for a long time it reflects conditions over months rather than at one instant.
Against that, it is qualitative: it tells you the water is polluted, not by how much and not with what. Other factors affect which species are present — temperature, acidity, the type of riverbed, whether a species ever reached the site at all — so an absence has more than one possible cause. A sensor or a chemical test gives a number and names the pollutant, but only for the moment it is taken.
Common mistake
An indicator species tells you about the conditions, not about the pollutant. "There were sludgeworms, so the water contains sewage" claims more than the evidence supports — it shows the oxygen level is low, and sewage is one of several things that could have caused that.
Before you practise
- All materials in the living world are recycled to provide the building blocks for future organisms.
- Carbon returns to the air through respiration and combustion, and is taken out of it by photosynthesis.
- Name the water cycle processes: evaporation, transpiration, condensation, precipitation.
- Decomposers are the part of a cycle answer most often left out — they return carbon as carbon dioxide and mineral ions to the soil.
- Nitrogen-fixing bacteria take nitrogen from the air; nitrifying bacteria turn ammonia into nitrates; denitrifying bacteria undo the work in waterlogged soil.
- Decay is fastest when it is warm, moist and oxygen is available — which is why food is preserved by doing the opposite.
- An indicator species tells you the conditions, not the pollutant.